Gibco™ Human CD142 Recombinant Protein, PeproTech®

Catalog No.	Quantity
15019T100UG	100 μg
15019T10UG	10 μg
150-19T-1MG	1 mg
15019T250UG	250 μg
150-19T-2UG	2 μg
15019T500UG	500 μg
15019T50UG	50 μg

Catalog No. 15019T100UG Supplier Gibco™ Supplier No. 15019T100UG

Description

Recombinant Protein

PeproTech's CHO cell-derived Recombinant Human Tissue Factor is a homodimeric glycoprotein comprised of the 227-amino-acid length monomer containing a C-terminal His-Tag and has a calculated molecular weight of 51.6 kDa. Due to glycosylation, Recombinant Human Tissue Factor migrates with an apparent molecular mass of approximately 40-50 kDa by SDS-PAGE gel under reducing conditions. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described.

Specifications

• Accession Number: P13726
• For Use With (Application): Control, Bioactivity
• Formulation: Protein with no preservative
• Gene ID (Entrez): 2152
• Molecular Weight (g/mol): 51.6 kDa
• Name: Human CD142
• Purification Method: Purified
• Quantity: 100 μg
• Source: CHO cells
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/μg
• Gene Alias: AA409063; CD142; Cf3; Cf-3; Coagulation factor III; coagulation factor III (thromboplastin, tissue factor); coagulation factor III, tissue factor; F3; TF; TFA; Thromboplastin; Tissue factor
• Common Name: CD142
• Gene Symbol: F3
• Biological Activity: Functional activity of Tissue Factor is determined by its ability to activate fluorogenic peptide substrate Boc-VPR-AMC cleavage, when bound in 1:1 complex with Coagulation Factor VII.
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: SGTTNTVAAY NLTWKSTNFK TILEWEPKPV NQVYTVQIST KSGDWKSKCF YTTDTECDLT DEIVKDVKQT YLARVFSYPA GNVESTGSAG EPLYENSPEF TPYLETNLGQ PTIQSFEQVG TKVNVTVEDE RTLVRRNNTF LSLRDVFGKD LIYTLYYWKS SSSGKKTAKT NTNEFLIDVD KGENYCFSVQ AVIPSRTVNR KSTDSPVECM GGEKGEFREH HHHHHHH
• Content And Storage: -20°C
• Activity: Functional activity of Tissue Factor is determined by its ability to activate fluorogenic peptide substrate Boc-VPR-AMC cleavage, when bound in 1:1 complex with Coagulation Factor VII.
• Expression System: CHO cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.