Invitrogen™ Human CD3 epsilon Heterodimer Recombinant Protein

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (200 μL) be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV.

CD3E and CD3D Heterodimer Protein is produced by mammalian expression system and are involved in mediating signal transduction.

Specifications

• Accession Number: P04234, P07766
• Concentration: 0.25 mg/mL
• For Use With (Application): ELISA
• Format: Lyophilized
• Formulation: PBS with 5% mannitol, 5% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 915, 916
• Molecular Weight (g/mol): 77.5 kDa
• Name: Human CD3 epsilon Heterodimer
• Purification Method: SDS-PAGE
• Quantity: 500 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/ μg
• Common Name: CD3e/CD3d
• Gene Symbol: CD3D, CD3E
• Biological Activity: Measured by its ability to bind OKT3-mIgG2a in a functional ELISA.
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: Human CD3D, Met1-Ala105 (Accession # P04234-1) with a C-terminal His-tag hIgG1 Fc region and human CD3E, Met1-Asp126 (Accession # P07766-1) with a C-terminal Flag-tag hIgG Fc region were co-expressed and purified as a human CD3D & CD3E heterodimer
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Measured by its ability to bind OKT3-mIgG2a in a functional ELISA.
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: ≥90% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.