Invitrogen™ Human CD30 Ligand/TNFSF8 ELISA Kit

Description

ELISA

Human CD30 Ligand/TNFSF8 quantitates human CD30 Ligand/TNFSF8 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human CD30 Ligand/TNFSF8.

The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that coexpress CD45RO and the IL4 receptor.

Specifications

• Accession Number: CD153; CD153 antigen; CD30 antigen ligand; CD30 ligand; Cd30l; CD30-L; CD30LG; CD30ligand; Tnfsf8; TNLG3A; tumor necrosis factor (ligand) superfamily member 8; tumor necrosis factor (ligand) superfamily, member 8; tumor necrosis factor ligand 3 A; tumor necrosis factor ligand superfamily member 8; tumor necrosis factor superfamily member 8
• Assay Range: 0.123 to 30 ng/mL
• Assay Sensitivity: 0.12 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 944
• Gene Symbol: Tnfsf8
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: CD153
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins