Invitrogen™ Human CD34 ELISA Kit

Catalog No.	Quantity
EH86RBX5	5 x 96 Tests
EH86RB	96 Tests

Catalog No. EH86RBX5 Supplier Invitrogen™ Supplier No. EH86RBX5

Description

ELISA

Human CD34 quantitates human CD34 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human CD34.

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

Specifications

• Accession Number: AU040960; Cd34; CD34 antigen; CD34 molecule; cluster designation 34; cluster of differentiation antigen 34; hematopoietic progenitor cell antigen CD34; hematopoietic progenitor cell marker CD34; LOC100343670; Mucosialin; RP11-328D5.2
• Assay Range: 0.82 to 200 ng/mL
• Assay Sensitivity: 0.05 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 947
• Gene Symbol: Cd34
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: CD34
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins