Gibco™ Human CD40 Ligand (TNFSF5) Recombinant Protein

Description

Recombinant Protein

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute sCD40L in 5-10 mM sodium phosphate, pH 7.5, to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C. Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage, preferably desiccated. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.

Specifications

• Accession Number: P29965
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: 10 mM sodium phosphate with no preservative
• Gene ID (Entrez): 959
• Molecular Weight (g/mol): 16.3 kDa
• Name: Human CD40 Ligand (TNFSF5)
• Purification Method: Sequential Chromatography
• Quantity: 50 μg
• Endotoxin Concentration: <0.1 ng/ μg
• Gene Alias: CD154; CD40 antigen ligand; CD40 ligand; CD40 ligand CD154; CD40 ligand, membrane form; CD40 ligand, soluble form; CD40L; CD40-L; Cd40lg; gp39; hCD40L; H-CD-40-L; HIGM1; IGM; IMD3; Ly62; Ly-62; M-CD-40-L; RP23-153G22.3; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TRAP; tumor necrosis factor (ligand) superfamily member 5; tumor necrosis factor (ligand) superfamily, member 5; tumor necrosis factor ligand superfamily member 5
• Common Name: CD154 (CD40 Ligand)
• Biological Activity: ED50 = 5 - 10 ng/mL; determined by the dose-dependent stimulation of IL-8 production by human PBMCs.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: Human CD40 ligand recombinant protein contains 149 amino acids
• Content And Storage: -20°C
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Other Proteins
• Research Category: Immunology, Stem Cell Research, Signal Transduction
• Product Line: Gibco™
• Purity or Quality Grade: ≥98% by SDS-PAGE and HPLC