Invitrogen™ Human CD51/Integrin alpha-V ELISA Kit

Catalog No.
EH92RBX10	10 x 96 Tests; HRP

Catalog No. EH92RBX10 Supplier Invitrogen™ Supplier No. EH92RBX10

Description

ELISA

Human CD51/Integrin alpha-V quantitates human CD51/Integrin alpha-V in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human CD51/Integrin alpha-V.

ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8, 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands, the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.

Specifications

• Accession Number: P06756
• Assay Range: 1.5-400 ng/mL
• Assay Sensitivity: 1.5 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 1110004F14Rik; 2610028E01Rik; alpha v integrin; antigen identified by monoclonal antibody L230; Cd51; CD61; D430040G12Rik; Dnmt3l-ps1 pseudogene; GP3A; GPIIIa; integrin alpha V; integrin alpha-V; Integrin alpha-V heavy chain; Integrin alpha-V light chain; integrin alphaVbeta3; integrin subunit alpha V; integrin, alpha V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); ITGAV; ITGB3; MSK8; Vitronectin receptor; vitronectin receptor alpha polypeptide (VNRA); vitronectin receptor subunit alpha; VNR; VNRA; VTNR1110004F14Rik; 2610028E01Rik; alpha v integrin; antigen identified by monoclonal antibody L230; Cd51; CD61; D430040G12Rik; Dnmt3l-ps1 pseudogene; GP3A; GPIIIa; integrin alpha V; integrin alpha-V; Integrin alpha-V heavy chain; Integrin alpha-V light chain; integrin alphaVbeta3; integrin subunit alpha V; integrin, alpha V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); ITGAV; ITGB3; MSK8; Vitronectin receptor; vitronectin receptor alpha polypeptide (VNRA); vitronectin receptor subunit alpha; VNR; VNRA; VTNR;
• Gene ID (Entrez): 3685
• Gene Symbol: ITGAV
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: Integrin alpha V (CD51)
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min