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Sino Biological Human CD59 Matched ELISA Antibody Pair Set 5 Plates
Supplier: Sino Biological SEK124745
The Human CD59 ELISA Pair Set is for the quantitative determination of Human CD59.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human CD59 coated on a 96-well plate. Standards and samples are added to the wells, and any Human CD59 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human CD59 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human CD59 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
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