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ACROBiosystems ACROBiosystems Human CD96 / TACTILE (C110S) Protein, His Tag (MALS verified)
Supplier: ACROBiosystems TAEH52H01MG
Recombinant Protein;1MG;Human CD96 (C110S), His Tag (TAE-H52H0) is expressed from human 293 cells (HEK293). It contains AA Val 22 - Met 503 (Accession # P40200-2 (C110S)).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 55.4 kDa. The protein migrates as 85-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human CD96 (C110S), His Tag (Cat. No. TAE-H52H0) at 5 μg/mL (100 μL/well) can bind Human CD155, Fc Tag (Cat. No. CD5-H5251) with a linear range of 39-156 ng/mL (QC tested).;FACS analysis of Human CD96, His Tag binding to CD155 cells overexpressing CD155.
2e5 of CD155 cells overexpressing CD155 were stained with 100 μL of 1 μg/mL of Human CD96 (C110S), His Tag (Cat. No. TAE-H52H0) and negative control protein respectively, washed and then followed by PE anti-His Tag antibody and analyzed with FACS (Routinely tested).
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