Invitrogen™ Human CHST4/GST-3 ELISA Kit

Description

ELISA

Human CHST4/GST-3 quantitates human CHST4/GST-3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human CHST4/GST-3.

This gene encodes an N-acetylglucosamine 6-O sulfotransferase. The encoded enzyme transfers sulfate from 3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. This protein is localized to the Golgi and is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein.

Specifications

• Accession Number: carbohydrate (chondroitin 6/keratan) sulfotransferase 4; carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4; carbohydrate sulfotransferase 4; CHST4; galactose/N-acetylglucosamine/N-acetylgalactosamine 6-O-sulfotransferase 3; galactose/N-acetylglucosamine/N-acetylglucosamine; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3; GlcNAc6ST2; GlcNAc6ST-2; Gn6st-2; Gst3; GST-3; HECGLCNAC6ST; HEC-GlcNAc6ST; high endothelial cell GlcNAC-6-sulphotransferase; high endothelial cells N-acetylglucosamine 6-O-sulfotransferase; L-selectin ligand sulfotransferase; LSST; N-acetylglucosamine 6-O-sulfotransferase 2
• Assay Range: 0.062 to 150 ng/mL
• Assay Sensitivity: 0.062 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 10164
• Gene Symbol: Chst4
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: CHST4
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov