Invitrogen™ Human CMASP-3 ELISA Kit

Description

ELISA

Human CMASP-3 quantitates human CMASP-3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human CMASP-3.

MBL is a key component in immune response in that it can directly trigger neutralization of invading microorganisms by an Ab-independent mechanism. Mutations of human MBL are associated with immunodeficiency resulting from a reduction in the ability of the mutant MBL to initiate complement fixation. In human, three types of MBL-associated serine proteases, MASP-1, MASP-2 and MASP-3, and a truncated form of MASP-2 (small MBL-associated protein; sMAP or MAp19) complex with MBL to activate the lectin pathway of the complement system. MASP-3 is an alternatively spliced product from the MASP-1 gene. The heavy/A chains are identical between MASP-1 and MASP-3 but the light/B chains are entirely different. Activated MASPs subsequently cleave and activate downstream components of the complement pathway.

Specifications

• Accession Number: 3 MC1; AW048060; C4/C2 activating component of Ra-reactive factor; CCPII; Complement factor MASP-3; complement-activating component of Ra-reactive factor; Crarf; CRARF1; mannan binding lectin serine peptidase 1; mannan-binding; mannan-binding lectin serine peptidase 1; mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor); mannan-binding lectin serine protease 1; Mannan-binding lectin serine protease 1 heavy chain; Mannan-binding lectin serine protease 1 light chain; Mannose-binding lectin-associated serine protease 1; mannose-binding protein associated serine protease-1; mannose-binding protein-associated serine protease; MAP1; MAp44; MASP; Masp1; MASP-1; Masp1/3; MASP3; PRSS5; Ra-reactive factor serine protease p100; RaRF; Serine protease 5; serine protease MASP1
• Assay Range: 150 to 0.6 ng/mL
• Assay Sensitivity: 0.6 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 5648
• Gene Symbol: MASP1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: MASP1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

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