Invitrogen™ Human CNTF R alpha ELISA Kit

Catalog No.
EH114RBX10	10 x 96 Tests; HRP

Catalog No. EH114RBX10 Supplier Invitrogen™ Supplier No. EH114RBX10

Description

ELISA

Human CNTF R alpha quantitates human CNTF R alpha in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human CNTF R alpha.

This gene encodes a hematopoeitin/interferon-class receptor belonging to the cytokine superfamily of receptors. The encoded gene product represents the CNTF-specific alpha subunit of a heterotrimer forming the CNTF receptor complex, which also includes LIFR and gp130. The receptor is attached to the membrane by a glycosyl-phosphatidylinositol linkage and contains an immunoglobulin-like C2-type domain and a fibronectin type-III domain. Signal transduction requires that CNTF bind first to this alpha component, which permits the recruitment of gp130 and LIFR beta to form the tripartite receptor complex. Signal transduction stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types. Alternative splicing has been observed at this locus and two variants, both encoding the same protein, have been identified.

Specifications

• Accession Number: P26992
• Assay Range: 12-3000 pg/mL
• Assay Sensitivity: 12 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: ciliary neurotrophic factor receptor; ciliary neurotrophic factor receptor alpha; ciliary neurotrophic factor receptor subunit alpha; CNTF receptor subunit alpha; Cntfr; CNTFR alpha; CNTFRALPHA; CNTFR-alpha; MGC1774ciliary neurotrophic factor receptor; ciliary neurotrophic factor receptor alpha; ciliary neurotrophic factor receptor subunit alpha; CNTF receptor subunit alpha; Cntfr; CNTFR alpha; CNTFRALPHA; CNTFR-alpha; MGC1774;
• Gene ID (Entrez): 1271
• Gene Symbol: Cntfr
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: CNTFR
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min