Invitrogen™ Human CRF ELISA Kit

Catalog No.	Quantity
EEL042	96 Tests

Catalog No. EEL042 Supplier Invitrogen™ Supplier No. EEL042

Description

ELISA

The Human Corticotropin Releasing Hormone (CRH) ELISA quantitates CRH in serum, plasma and other biological fluids Principle of the method: The Competitive Corticotropin Releasing Hormone (CRH) ELISA research-use-only kit is designed to quantitatively measure CRH in Humans. A CRH standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Human CRH is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Human CRH in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Corticotropin Releasing Factor (CRF), a 41 amino acids neuropeptide, belongs to a family of structurally related peptides including urocortin, sauvagine and urotensin I. CRF is critical in the regulation of the pituitary-adrenal gland axis, and in endocrine, autonomic and behavioral responses to stress. CRF is widely distributed in the brain and peripheral nervous system with the highest levels in the hypothalamic paraventricular nucleus.

Specifications

• Accession Number: P06850
• Assay Range: 3.91-250 pg/mL
• Assay Sensitivity: 2.35 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Sauvagine/corticotropin-releasing factor/urotensin I
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Corticoliberin; corticotropin releasing factor; corticotropin releasing hormone; corticotropin releasing hormone receptor 2; corticotropin-releasing factor; Corticotropin-releasing factor receptor 2; corticotropin-releasing hormone; Corticotropin-releasing hormone receptor 2; CRF; CRF2; CRF2 receptor, beta isoform; CRF2R; CRFR2; CRF-R-2; CRF-RB; CRH; CRH receptor 2 variant B; CRH1; CRH2R; CRHR2; CRH-R2; CRH-R-2; Gm1347; HM-CRFCorticoliberin; corticotropin releasing factor; corticotropin releasing hormone; corticotropin releasing hormone receptor 2; corticotropin-releasing factor; Corticotropin-releasing factor receptor 2; corticotropin-releasing hormone; Corticotropin-releasing hormone receptor 2; CRF; CRF2; CRF2 receptor, beta isoform; CRF2R; CRFR2; CRF-R-2; CRF-RB; CRH; CRH receptor 2 variant B; CRH1; CRH2R; CRHR2; CRH-R2; CRH-R-2; Gm1347; HM-CRF;
• Gene ID (Entrez): 1392
• Gene Symbol: CRH
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: CRF
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min