Sigma Aldrich Fine Chemicals Biosciences Human CRISPRi Common Cance

Supplier:  Sigma Aldrich Fine Chemicals Biosciences CRISPRIS101KT

CRISPRi is a potent tool for modulating gene expression. This technology uses dCas9 to act as a synthetic transcription factors by recruiting endogenous transcription repressor complexes to gene promoters and enhancers resulting in down regulated gene transcription. CRISPRi achieves LOF phenotypes without the limitations of RNAi and CRISPR KO although CRISPRi relies on delivery by lentivirus this technology offers new possibilities for genome engineering. CRISPRi can be used individually to target areas of the genome that are inaccessible by other gene perturbation technologies (e.g. non-coding regions) or in conjunction to uncover gene-regulatory networks underlying discrete phenotypes. Finally pooled CRISPRi screening can be paired with single-cell RNA-sequencing methods to enable high-dimensional characterization of CRISPR gene perturbation. Human CRISPRi Common Cancer Cell Lines Kit contains 1 sub-pools of top 5 gRNAs per gene as well as a separate sub-pool of 5 supplemental g

Catalog No. 50-290-8857