Invitrogen™ Human CTGF ELISA Kit

Catalog No.	Quantity
EEL036	96 Tests

Catalog No. EEL036 Supplier Invitrogen™ Supplier No. EEL036

Description

ELISA

The Human Connective Tissue Growth Factor (CTGF) ELISA quantitates CTGF in serum, plasma, and other biological fluids The Human Connective Tissue Growth Factor solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Connective Tissue Growth Factor (CTGF), also called CCN2, is a member of the CCN family. CTGF is a secreted protein produced by umbilical veins and vascular endothelial cells. CTGF possesses an Insulin-like Growth Factor (IGF)-binding domain, a thrombospondin type 1 domain, and a cysteine knot region. CTGF plays important roles in the proliferation and differentiation of chondrocytes, induces angiogenesis, and promotes cell adhesion in fibroblasts, endothelial, and epithelial cells.

Specifications

• Accession Number: P29279
• Assay Range: 62.5-4000 pg/mL
• Assay Sensitivity: 37.5 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: CCN
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 2-Nov; betaIG-M2; CCN family member 2; Ccn2; Cellular communication network factor 2; connective tissue growth factor; connective tissue growth factor precursor; Connective tissue growth factor precursor (CTGF) (FISP-12 protein) (Hypertrophic chondrocyte-specific protein 24); connective tissue growth-related protein; CTGF; CTGF protein; CTGRP; fibroblast inducible secreated protein; fibroblast inducible secreted protein; Fisp12; fisp-12; Hcs24; H-CTGF; hypertrophic chondrocyte-specific gene product 24; hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP8; IGFBP-8; Insulin-like growth factor-binding protein 8; MGC102839; NOV2; Protein FISP-12; similar to connective tissue growth factor precursor2-Nov; betaIG-M2; CCN family member 2; Ccn2; Cellular communication network factor 2; connective tissue growth factor; connective tissue growth factor precursor; Connective tissue growth factor precursor (CTGF) (FISP-12 protein) (Hypertrophic chondrocyte-specific protein 24); connective tissue growth-related protein; CTGF; CTGF protein; CTGRP; fibroblast inducible secreated protein; fibroblast inducible secreted protein; Fisp12; fisp-12; Hcs24; H-CTGF; hypertrophic chondrocyte-specific gene product 24; hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP8; IGFBP-8; Insulin-like growth factor-binding protein 8; MGC102839; NOV2; Protein FISP-12; similar to connective tissue growth factor precursor;
• Gene ID (Entrez): 1490
• Gene Symbol: CCN2
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: CTGF
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min