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ACROBiosystems Human CTLA-4 / CD152 Protein, His Tag, active dimer (MALS verified)

Supplier:  ACROBiosystems CT4H52H9100UG

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Recombinant Protein;100UG;Human CTLA-4, His Tag, active dimer (CT4-H52H9) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162 (Accession # Q6GR94-1).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 18.8 kDa. The protein migrates as 23-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (Cat. No. CT4-H52H9) with a linear range of 0.1-2 ng/mL (QC tested).;Immobilized Human CTLA-4, His Tag, active dimer (Cat. No.CT4-H52H9) at 2 μg/mL, add increasing concentrations of CTLA-4 x OX40 Bispecific Antibody in 50% Human serum and then add Biotinylated Human OX40, Avitag,His Tag(Cat. No. TN4-H82E4) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Intact assay, Routinely tested).

Catalog No. 50-205-5070


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