Invitrogen™ Human CYB561D2 (aa 1-21) Control Fragment Recombinant Protein

Catalog No.	Quantity
RP100132	100 μL

Catalog No. RP100132 Supplier Invitrogen™ Supplier No. RP100132

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (95%), Rat (95%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-60137. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

CYB561D2 (Cytochrome b561 domain-containing protein 2), also known as putative tumor suppressor protein 101F6, is a 222 amino acid highly hydrophobic protein that contains six membrane spanning domains. Localized to endosomes and endoplasmic reticulum of the perinuclear region, CYB561D2 contains 2 heme-binding sites, through which it reduces ferric ions. CYB561D2 is ubiquitously expressed, with highest levels in lung, liver and kidney. Though expression of CYB561D2 is abundant in normal lung bronchial epithileal cells, CYB561D2 is absent in most lung cancers. The gene encoding CYB561D2 maps to human chromosome 3p21.3, a region that is prone to genetic alterations and is associated with frequent and early allele loss in many human cancers. Exogenous expression of CYB561D2 leads to the induction of caspase-independent apoptotic and autophagic pathways, resulting in the synergistic killing of tumor cells. This suggests an important role for CYB561D2 as a therapeutic intervention in cancer treatment.

Specifications

• Accession Number: O14569
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Neutralization, Control
• Formulation: PBS, 1M urea with no preservative; pH 7.4
• Gene ID (Entrez): 11068
• Name: Human CYB561D2 (aa 1-21) Control Fragment
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 100 μL
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 101F6; 101F6 protein; AW049681; CYB561D2; cytochrome b-561 domain containing 2; cytochrome b561 domain-containing protein 2; cytochrome b561 family member D2; cytochrome b561 family, member D2; gene for 101F6 protein; LUCA12.2; OTTHUMP0000021067; OTTHUMP00000210675; OTTHUMP00000210676; Putative tumor suppressor protein 101F6; Transmembrane ascorbate ferrireductase; Tsp10; tumor suppressor, region 10; XXcos-LUCA11.4
• Common Name: CYB561D2
• Gene Symbol: CYB561D2
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: MALSAETESHIYRALRTASGA
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining