Invitrogen™ Human Cystatin E/M ELISA Kit

Catalog No.	Quantity
EH142RBX5	5 x 96 Tests
EH142RBX10	10 x 96 Tests
EH142RB	96 Tests

Catalog No. EH142RBX5 Supplier Invitrogen™ Supplier No. EH142RBX5

Description

ELISA

Human Cystatin E/M quantitates human Cystatin E/M in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Cystatin E/M.

The cystatin superfamily is a well-established family of cysteine protease inhibitors. Cystatins A and B (type 1) are mainly intracellular; cystatins C, D, E/M, F, G, S, SN and SA cystatins are extracellular (type 2); and the kininogens are type 3 cystatins which are intravascular proteins. All true cystatins inhibit cysteine peptidases of the papain family, such as cathepsins, and some also inhibit legumain family enzymes. Cystatin SA, cystatin S and cystatin SN are found primarily in saliva. Cystatin S and SN can also be expressed in tears, urine and seminal fluid. Cystatin C is a related protein which is expressed in brain, thymus, ovary, epididymis and vas deferens. Cystatin D protects against proteinases in the oral cavity, while Cystatin E/M and F moderate the inhibition of cathepsin proteins. The fetuins, part of the cystatin superfamily, are secretable proteins that influence osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors and the response to systemic inflammation. High molecular weight kininogen (Kininogen HC) and low molecular weight kininogen (Kininogen LC) have varied roles, though they both inhibit the thrombin- and plasmin-induced aggregation of thrombocytes.

Specifications

• Accession Number: Q15828
• Assay Range: 0.14-30 ng/mL
• Assay Sensitivity: 0.14 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 1110017E11Rik; AA960480; CST6; Cstb; cystatin 6; cystatin B; cystatin E/M; cystatin M; cystatin M/E; cystatin N; cystatin-6; Cystatin-B; cystatin-E; Cystatin-M; cysteine proteinase inhibitor; harlequin ichthyosis; harlequin ichthysosis; ichq; N28197; Stefin-B; Stfb1110017E11Rik; AA960480; CST6; Cstb; cystatin 6; cystatin B; cystatin E/M; cystatin M; cystatin M/E; cystatin N; cystatin-6; Cystatin-B; cystatin-E; Cystatin-M; cysteine proteinase inhibitor; harlequin ichthyosis; harlequin ichthysosis; ichq; N28197; Stefin-B; Stfb;
• Gene ID (Entrez): 1474
• Gene Symbol: CST6
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: Cystatin M
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov