Invitrogen™ Human D-Dimer Rapid ELISA Kit

Catalog No.	Quantity
EELR023	96 Tests

Catalog No. EELR023 Supplier Invitrogen™ Supplier No. EELR023

Description

ELISA

The Human D Dimer (D2D) ELISA quantitates D2D in plasma. Principle of the method The Human D2D solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells, where Human D2D in the samples (or standards) binds to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) HRP linked antibody specific for Human D2D. Excess conjugate and unbound sample or standard are washed away from the plate. The substrate solution is added to each well, and the enzyme substrate reaction is terminated by the addition of a stop solution, turning the color yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The intensity of this signal is directly proportional to the concentration of Human D2D present in the original specimen, which is determined by comparing the OD of the samples to the standard curve. Achieve fast and reliable results in just 90 minutes with our Rapid ELISA kit, featuring a streamlined protocol that requires only one wash step. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For...

Fibrinogen is the main protein of blood coagulation system. It consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. The process of blood coagulation results in the activation of fibrinogen into fibrin by thrombin and fibrin polymerization. Fibrin clot is then digested by plasmin, and fibrin degradation products of different molecular weights are released into the bloodstream.

Specifications

• Accession Number: P02671
• Assay Range: 125-4000 pg/mL
• Assay Sensitivity: 53.1 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: carboxy-terminal region; ENSMUSG00000059807; FGA; Fib; Fib2; fibrinogen A-alpha-chain; fibrinogen alpha chain; fibrinogen, A alpha polypeptide; Fibrinopeptide A; MGC119422; MGC119423; MGC119425carboxy-terminal region; ENSMUSG00000059807; FGA; Fib; Fib2; fibrinogen A-alpha-chain; fibrinogen alpha chain; fibrinogen, A alpha polypeptide; Fibrinopeptide A; MGC119422; MGC119423; MGC119425;
• Gene ID (Entrez): 2243
• Gene Symbol: FGA
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: D-dimer
• Test Time: 15 min
• Total Assay Time: 1 hr 30 min