Gibco™ Human Dermal Fibroblasts, adult (HDFa)

Catalog No.	Quantity
C0135C	1 vial

Catalog No. C0135C Supplier Gibco™ Supplier No. C0135C

Description

Human Dermal Fibroblasts, adult (HDFa)

Primary human dermal fibroblasts isolated from adult skin, cryopreserved at the end of the primary culture.

Viability at least 70%

≥500,000 viable cells/vial

Capable of at least 12 population doublings

Mycoplasma: Not Detected

Hepatitis B: Not Detected

Hepatitis C: Not Detected

HIV-1: Not Detected

Bacteria, yeast, and other fungi: Not Detected

Specifications

• Content And Storage: Store in liquid nitrogen.
• Age: Adult
• Cell Type: Fibroblast Cells (Dermal)
• Culture Type: Adherent Cell Culture
• Form: Cryopreserved
• Species: Human
• Donor Source: Single Donor
• No. of Cells: ≥500000
• Product Line: Cascade Biologics
• Product Type: Human Fibroblasts
• Quantity: 1 vial
• Tested For: Hepatitis B (HBV), Hepatitis C (HCV), Sterility Testing (Bacteria & Fungi), Mycoplasma, Growth Performance Assay, HIV-1

Frequently Asked Questions (FAQs)

Can I get passage information for Human Dermal Fibroblasts, adult (HDFa) (Cat. No. C0135C) and Human Dermal Fibroblasts, neonatal (HDFn) (Cat. No. C0045C)?

Human Dermal Fibroblasts, adult (HDFa) (Cat. No. C0135C) and Human Dermal Fibroblasts, neonatal (HDFn) (Cat. No. C0045C) are primary human dermal fibroblasts isolated from adult skin and neonatal foreskin, respectively. They are cryopreserved at the end of the primary culture stage and not passaged.

What are your Human Dermal Fibroblast (HDF) cells grown in?

HDF cells are grown in Medium 106 with the addition of Low Serum Growth Supplement.

What media do you use during the first passage of primary cells?

We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).

How is the population doubling calculated for primary cells?

Cumulative population doublings are calculated from the seeding density and harvesting density from each culture level.

What are typical primary cell yields from tissue?

It varies a lot depending on the age of donor, size of tissues, locations, and conditions arrived to our facility. Sometime a small amount of tissue gives us many cells and sometimes a large amount of tissue gives us a very small lot. Typically, we can get 30-300 vials.

Are primary cells isolated from tissue using enzymatic digestion?

Yes, our primary cells are isolated from tissue using enzymatic digestion.

What are primary cells?

Primary cells are cells taken directly from living tissue (e.g., biopsy material) and established for growth in vitro. These cells have undergone very few population doublings and are therefore more representative of the main functional component of the tissue from which they are derived in comparison to continuous (tumor or artificially immortalized) cell lines, making primary cells a more representative model for the in vivo state.

Primary cells from different species may be used, allowing you to highlight potential differences between humans and preclinical test species. Before in vivo studies, mouse or rat cells can be used to refine doses and reduce the number of animals required for preclinical toxicology. Human cells can be used to determine the accuracy of extrapolating human data from an animal model.

For Research Use Only. Not for use in diagnostic procedures.