Invitrogen™ Human Desmin ELISA Kit

Catalog No.	Quantity
EH152RBX5	5 x 96 Tests
EH152RBX10	10 x 96 Tests
EH152RB	96 Tests

Catalog No. EH152RBX5 Supplier Invitrogen™ Supplier No. EH152RBX5

Description

ELISA

Human Desmin quantitates human Desmin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Desmin.

Desmin (approximately 53 kDa) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.

Specifications

• Accession Number: CMD1I; CSM1; CSM2; Des; desmin; desmin-like protein; FLJ12025; FLJ39719; FLJ41013; FLJ41793; I79
• Assay Range: 2 to 500 ng/mL
• Assay Sensitivity: 2 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 1674
• Gene Symbol: DES
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Desmin
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins