Invitrogen™ Human Desmoglein-3 ELISA Kit

Catalog No.	Quantity
EH155RBX5	5 x 96 Tests
EH155RB	96 Tests
EH155RBX10	10 x 96 Tests

Catalog No. EH155RBX5 Supplier Invitrogen™ Supplier No. EH155RBX5

Description

ELISA

Human Desmoglein-3 quantitates human Desmoglein-3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Desmoglein-3.

Desmoglein 3 (Dsg3), also known as Cadherin family member 6 (CDHF6), is a member of the desmosomal cadherin family and plays a critical role in cell-cell adhesion. It is predominately expressed in stratified squamous epithelium, including the skin, oral mucosa, and esophagus. Desmoglein 3 has been characterized as the auto-antigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease. Binding of anti-Desmoglein 3 autoantibodies causes the loss of cell-cell adhesion, which clinically manifests as blisters on the skin and oral mucosa, and histologically as suprabasilar acantholysis.

Specifications

• Accession Number: 130 kDa pemphigus vulgaris antigen; 130 kDa pemphigus vulgaris antigen homolog; bal; balding; Cadherin family member 6; CDHF6; desmoglein 3; desmoglein-3; DKFZp686P23184; Dsg3; PVA
• Assay Range: 34 to 8,000 pg/mL
• Assay Sensitivity: 34 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 1830
• Gene Symbol: Dsg3
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Desmoglein 3
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins