MilliporeSigma™ Human Dopaminergic Neurogenesis Kit

Description

Contains necessary growth factors and complete differentiation media needed to differentiate human ES/iPS cells into dopaminergic neurons using 21 day differentiation protocol. MilliporeSigma™; Human Dopaminergic Neurogenesis Kit has been validated with 4 human ES and iPS lines for efficient dopaminergic neuronal differentiation.

• Contains collection of five validated growth factors that are routinely used to induce differentiation of human pluripotent ES/iPS cells to dopaminergic neurons 
• Has high affinity antibody marker, anti-tyrosine hydroxylase, to aid in quantifying percentage yields from differentiation experiments
• H1: 30% TH positive neurons
• H9: 50-70% TH positive neurons
• Two Human iPS cell lines: ∼ 50% TH positive neurons
• Components:
• 2 vials of human recombinant Sonic Hedgehog (Shh) (5ug/vial)
• 1 vial of human recombinant FGF-8 (5ug/vial)
• 1 vial of human recombinant BDNF (2ug/vial)
• 1 vial of human recombinant GDNF (2ug/vial)
• 1 vial of human recombinant TGF-b-III (2ug/vial)
• 1 vial of anti-tyrosine hydroxylase, rabbit polyclonal (20uL)

Specifications

• Product Type: Neurogenesis Kit
• For Use With (Application): Cell Differentiation, Stem Cell Culture, Cell Culture, Neurotoxicity, Neuroscience
• Quantity: 225 mL Media and Growth Factor Kit
• Regulatory Status: RUO
• Content And Storage:
  • Human ES/iPS Neural Induction Medium: (Catalog No. SCM110)
  • Human ES/iPS Neuronal Differentiation Medium: (Catalog No. SCM111)
  • Dopaminergic Differentiation Growth Factor Sampler (Cat. No. SCR128)
  All components within SCR135 (Cat. No. SCM110, SCM111 and SCR128) should be stored at -20°C upon receiving.