Invitrogen™ Human EGFR Fc Chimera Recombinant Protein

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (4000 μL) be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV.

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

Specifications

• Accession Number: P00533
• For Use With (Application): ELISA
• Format: Lyophilized
• Formulation: PBS with 0.01% Tween 80, 5% mannitol, 5% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 1956
• Molecular Weight (g/mol): 110-140 kDa
• Name: Human EGFR Fc Chimera
• Purification Method: SDS-PAGE
• Quantity: 1 mg
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: 2.7.10.1; 9030024J15Rik; AI552599; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; egf receptor; Egfr; EGFR-related peptide; epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); ERBB; ERBB1; ErbB-1; erb-b2 receptor tyrosine kinase 1; Errb1; Errp; HER1; kinase EGFR; mENA; NISBD2; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; Urogastrone; wa2; wa-2; Wa5; waved 2
• Common Name: EGFR
• Gene Symbol: EGFR
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human EGF at 10 μg/ml (100 μL/well) can bind human EGFR with a linear range of 0.64-400 ng/mL
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: Human EGFR, amino acids Met1-Ser645 (Accession # NP_005219) with a C-terminal human IgG1 Fc region
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human EGF at 10 μg/ml (100 μL/well) can bind human EGFR with a linear range of 0.64-400 ng/mL
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >97% as determined by SDS-PAGE. >85% as determined by SEC-HPLC.