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ACROBiosystems Recombinant Protein;Human EGF R Protein;HEK293;100UG;EGR,EGFR,EGF R,ERBB,ERBB1,HER1,PIG61,mENA,EGF-R,ERBB-1,HER-1,EGFRvIII
Supplier: ACROBiosystems EGRH5222100UG
Recombinant Protein;Human EGF R Protein;100UG;Human EGF R, His Tag (EGR-H5222) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 70.5kDa. The protein migrates as 95-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Human EGF R, His Tag (Cat. No. EGR-H5222) with a linear range of 0.8-6 ng/mL (QC tested).;Erbitux (Cetuximab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human EGF R, His Tag (Cat. No. EGR-H5222) with an affinity constant of 1.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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