Invitrogen™ Human EN-RAGE/S100A12 ELISA Kit

Description

ELISA

Human EN-RAGE/S100A12 quantitates human EN-RAGE/S100A12 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human EN-RAGE/S100A12.

S100A12 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A12 is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. S100A12 is also a ligand for RAGE. Interaction with RAGE on mononuclear phagocytes, lymphocytes and endothelium triggers cellular activation. Nuclear factor kappa B, a key transcription factor for inflammatory events, is activated by S100A12/RAGE. S100A12 has also been shown to have anti-microbial properties.

Specifications

• Accession Number: CAAF1; CAGC; Calcitermin; calcium-binding protein in amniotic fluid 1; calgranulin C; calgranulin-C; CGRP; EN RAGE; ENRAGE; EN-RAGE; Extracellular newly identified RAGE-binding protein; Migration inhibitory factor-related protein 6; MRP6; MRP-6; Neutrophil S100 protein; p6; Protein S100 A12; Protein S100-A12; S100 calcium binding protein A12; S100 calcium-binding protein A12; S100 calcium-binding protein A12 (calgranulin C); S100A12
• Assay Range: 0.82 to 200 ng/mL
• Assay Sensitivity: 0.82 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 6283
• Gene Symbol: S100A12
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: S100A12
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins