Gibco™ Human Epidermal Melanocytes, neonatal, moderately pigmented donor, (HEMn-MP)

Catalog No.	Quantity
C1025C	1 vial

Catalog No. C1025C Supplier Gibco™ Supplier No. C1025C

Description

Primary human epidermal melanocytes isolated from neonatal foreskin, cryopreserved at the end of the secondary culture. These cells are isolated from moderately pigmented tissue.

Primary human epidermal melanocytes isolated from neonatal foreskin, cryopreserved at the end of the secondary culture. These cells are isolated from moderately pigmented tissue.

• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 12 population doublings

Tested for

• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected

Specifications

• Content And Storage: Liquid nitrogen temperature
• Age: Newborn (14 days or less)
• Cell Type: Melanocyte Cells
• Culture Type: Adherent Cell Culture
• Form: Cryopreserved
• Species: Human
• Donor Attributes: Moderately Pigmented
• Donor Source: Single Donor
• No. of Cells: ≥500,000
• Product Line: Cascade Biologics
• Product Type: Human Epidermal Melanocyte
• Quantity: 1 vial
• Tested For: Hepatitis B (HBV), Hepatitis C (HCV), Sterility Testing (Bacteria and Fungi), Mycoplasma, Growth Performance Assay, HIV-1

Frequently Asked Questions (FAQs)

Do you have ethnicity information for your human epidermal melanocyte donor lines?

We do provide age/sex/pigmentation/ethnicity on the Certificate of Analysis (COA).

How should melanocytes be frozen?

Do not use Synth-a-Freeze Cryopreservation Medium to cryopreserve melanocytes. We recommend using DMEM with 10% FBS and 10% DMSO, or the Recovery Cell Culture Freezing Medium.

What media do you use during the first passage of primary cells?

We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).

How is the population doubling calculated for primary cells?

Cumulative population doublings are calculated from the seeding density and harvesting density from each culture level.

What are typical primary cell yields from tissue?

It varies a lot depending on the age of donor, size of tissues, locations, and conditions arrived to our facility. Sometime a small amount of tissue gives us many cells and sometimes a large amount of tissue gives us a very small lot. Typically, we can get 30-300 vials.

Are primary cells isolated from tissue using enzymatic digestion?

Yes, our primary cells are isolated from tissue using enzymatic digestion.

What are primary cells?

Primary cells are cells taken directly from living tissue (e.g., biopsy material) and established for growth in vitro. These cells have undergone very few population doublings and are therefore more representative of the main functional component of the tissue from which they are derived in comparison to continuous (tumor or artificially immortalized) cell lines, making primary cells a more representative model for the in vivo state.

Primary cells from different species may be used, allowing you to highlight potential differences between humans and preclinical test species. Before in vivo studies, mouse or rat cells can be used to refine doses and reduce the number of animals required for preclinical toxicology. Human cells can be used to determine the accuracy of extrapolating human data from an animal model.

For Research Use Only. Not for use in diagnostic procedures.