Invitrogen™ Human EPO ELISA Kit

Description

ELISA

The Human EPO ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of human EPO.

Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.

Specifications

• Accession Number: EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2
• Assay Range: 1.6 to 100
• Assay Sensitivity: 0.14 mIU/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 2056
• Gene Symbol: EPO
• Interassay CV: 4.3%
• Intraassay CV: 6.2
• Kit Contents: Pre-coated 96 well plate, Standard (2 vials), Assay Buffer concentrate, Biotinylated Detection Antibody, Streptavidin-HRP, Wash Buffer, Substrate Solution, Stop Solution, Adhesive Plate Covers, Control High, Control Low
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Epo
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 30 mins