Gibco™ Human EPO (Erythropoietin) Recombinant Protein

Description

Recombinant Protein

Carrier-Free Reconstitution: Centrifuge the vial briefly, prior to opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile water to a concentration of 0.1-1 mg/mL to regain full activity. Apportion the reconstituted protein into working aliquots and store at ≤-20°C. Make any further dilutions of the reconstituted protein in medium or buffered solution containing carrier protein, such as PBS with 0.1% BSA. Storage: Store lyophilized human EPO 2°C to 8°C, preferably desiccated. Store reconstituted human EPO at ≤-20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.

Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.

Specifications

• Accession Number: P01588
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 2056
• Molecular Weight (g/mol): 18.4 kDa
• Name: Human EPO (Erythropoietin)
• Purification Method: Sequential Chromatography
• Quantity: 10 μg
• Endotoxin Concentration: <0.1 ng/ μg
• Gene Alias: EP; EPO; Epoetin; erythropoietin; MGC138142; MVCD2
• Common Name: Epo
• Biological Activity: ED50 ≤ 0.8 ng/mL; determined by the dose-dependent proliferation of TF-1 cells.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Content And Storage: 4°C
• Classification: Carrier-Free
• Activity: 0.8 ng/ml
• Bioassay: Proliferation of TF1 cells
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Wet Ice
• Expression System: CHO cells
• Protein Family: Growth Factors & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: EPO
• Research Category: Stem Cell Research, Neurobiology
• Product Line: Gibco™
• Purity or Quality Grade: ≥95% by SDS-PAGE

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov