Gibco™ Human EPO (Erythropoietin) Recombinant Protein

Description

Recombinant Protein

Carrier-Free Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile, deionized water to a concentration of 12,000-120,000 IU/mL. Further dilutions can be made in aqueous buffers. For long term storage, EPO solutions should be supplemented with carrier protein, such as 0.1% HSA or BSA. Storage: This lyophilized preparation is stable at room temperature for three weeks, but should be stored desiccated below -18°C. Upon reconstitution, the preparation is stable for up to one week at 2°C to 8°C, but should be stored below -18°C for long term storage. Avoid repeated freeze/thaw cycles.

Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.

Specifications

• Accession Number: P01588
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: 0.006 mg citric acid, 0.59 mg sodium citrate with 0.58 mg NaCl and no preservative
• Gene ID (Entrez): 2056
• Molecular Weight (g/mol): 30.4 kDa
• Name: Human EPO (Erythropoietin)
• Purification Method: Sequential Chromatography
• Quantity: 500 IU
• Endotoxin Concentration: <0.1 ng/μg
• Gene Alias: EP; EPO; Epoetin; erythropoietin; MGC138142; MVCD2
• Common Name: Epo
• Biological Activity: Specific Activity = 150,000 IU/mg; determined by measuring normocythaemic mice.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR
• Content And Storage: -20°C
• Classification: Carrier-Free
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Expression System: CHO cells
• Protein Family: Growth Factors & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: EPO
• Research Category: Neurobiology, Stem Cell Research, Differentiation
• Product Line: Gibco™
• Purity or Quality Grade: >98% by SDS-PAGE

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov