Invitrogen™ Human ErbB2 Fc Chimera Recombinant Protein

Catalog No.	Quantity
A42494	50 μg
A42493	5 μg

Catalog No. A42494 Supplier Invitrogen™ Supplier No. A42494

Description

Recombinant Protein

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.

Specifications

• Accession Number: P04626
• For Use With (Application): Control, Binding Assay, Bioactivity, Western Blot
• Formulation: PBS with 5% mannitol, 5% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Gene ID (Entrez): 2064
• Molecular Weight (g/mol): 96.1 kDa
• Name: Human ErbB2 Fc Chimera
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 50 μg
• Source: HEK293 cells
• Storage Requirements: -20°C or -80°C if preferred
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: avian erythroblastosis oncogene B 2; Avian erythroblastosis viral (v-erb-B2) oncogene homologue 2 (neuro/glioblastoma derived oncogene homolog); CD antigen CD340; CD340; c-erb B2/neu protein; c-erbB2; C-erbB-2; c-neu; epidermal growth factor receptor-related protein; erbb 2; ERBB2; erb-b2; Erbb-2; ErbB2 (pTyr1139); ErbB2 (pY1139); ErbB2 phospho Y1139; erb-b2 receptor tyrosine kinase 2; her 2; HER2; HER-2; HER-2/neu; herstatin; human epidermal growth factor receptor 2; Kiaa3023; metas; Metastatic lymph node gene 19 protein; mKIAA3023; MLN 19; MLN19; NEU; Neu oncogene; NEU proto-oncogene; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; NGL; p185erbB2; p185neu; phospho ErbB2; proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Common Name: Her-2 (ErbB2)
• Gene Symbol: ERBB2
• KinaseFamily: EGF Receptor (ErbB) Kinase Family
• Biological Activity: 1. Immobilized Human ErbB2 Fc Chimera Recombinant Protein (at 2 μg/mL (100 μL/well) can bind Anti-Erbb2 Antibody (Trastuzumab), the EC50 is 16-80 ng/mL 2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 μg/mL in the presence of 0.6 μg/mL Anti-ErbB2/Her2 Monoclonal Antibody.)
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: Human ErbB2, amino acids Met1-Thr652 (Accession # NP_004439.2) with a C-terminal Fc-tag
• Content And Storage: Includes 1 vial of lyophilized protein. Store at -20 °C.
• Activity: 1. Immobilized Human ErbB2 Fc Chimera Recombinant Protein at 2 μg/mL (100 μL/well) can bind Anti-Erbb2 Antibody (Trastuzumab), the EC50 is 16-80 ng/mL.<br><br>2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 μg/mL in the presence of 0.6 μg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
• Endotoxin Level: < 1 EU/μg
• Kinase Group: Tyrosine Kinases
• Expression System: HEK293 cells
• Protein Family: Growth Factors & Receptors
• Protein Form: Cell Surface Protein, Recombinant, Truncated
• Form: Lyophilized
• Protein Subtype: Growth Factor Receptors, Receptor Tyrosine Kinases
• Research Category: Cardiovascular Research, Immunology, Oncology, Signal Transduction, Stem Cell Research
• Purity or Quality Grade: ≥90 % as determined by SDS-PAGE. ≥90 % as determined by SEC-HPLC.
• Protein: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2