Invitrogen™ Human ESAM ELISA Kit

Description

ELISA

Human ESAM quantitates human ESAM in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human ESAM.

ESAM is a 55-kDa membrane protein composed of 2 extracellular Ig domains, a single transmembrane domain, and a 120 amino acid cytoplasmic domain. ESAM is related to members of the junctional adhesion molecule (JAM) family, and is expressed on endothelial cells and activated platelets. ESAM at endothelial tight junctions plays a role in the regulation of vascular permeability, in response to agents such as VEGF or extravasating neutrophils.

Specifications

• Accession Number: 2310008D05Rik; endothelial cell adhesion molecule; endothelial cell-selective adhesion molecule; endothelial cell-specific adhesion molecule; ESAM; Esam1; HUEL (C4orf1)-interacting protein; LP4791 protein; UNQ220/PRO246; W117m
• Assay Range: 0.082 to 20 ng/mL
• Assay Sensitivity: 0.08 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 90952
• Gene Symbol: ESAM
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: ESAM
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins