Invitrogen™ Human FABP-5 ELISA Kit

Description

ELISA

Human FABP-5 quantitates human FABP-5 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human FABP-5.

FABP5 (fatty acid binding protein 5 (psoriasis-associated)) is a proteinthat in humans is encoded by theFABP5 gene, also known as PAFABP, EFABP, E-FABP, KFABP, PA-FABP, Epidermal-type fatty acid-binding protein, Fatty acid-binding protein 5, Psoriasis-associated fatty acid-binding protein homolog. It is a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. The PAFABP cDNA encodes a 135-amino acid protein with molecular weight 15,164. This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated inpsoriasistissue. It is though that FABPs roles include fatty acid uptake, transport, and metabolism. Kaczocha et al. (2009) identifiedFABP5and FABP7 as cytosolic proteins that transport AEA from the plasma membrane to subcellular fatty acid amide hydrolase, where it is hydrolyzed and inactivated. FABP3 did not show this specific transport function.

Specifications

• Accession Number: C-FABP; Cutaneous fatty acid-binding protein; DA11; EFABP; E-FABP; E-FABPPAFABP; Epidermal-type fatty acid-binding protein; epithelial fatty acid-binding protein; FABP5; Fabpe; fatty acid binding protein 5; fatty acid binding protein 5 (psoriasis-associated); fatty acid binding protein 5, epidermal; fatty acid-binding protein 5; Fatty acid-binding protein, epidermal; fatty acid-binding protein, epidermal; uncharacterized protein LOC16592; Keratinocyte lipid-binding protein; KFABP; Klbp; Mal1; PAFABP; PA-FABP; PA-FABPepidermal; psoriasis-associated fatty acid-binding protein homolog
• Assay Range: 12.5 to 800 ng/mL
• Assay Sensitivity: 12.5 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 2171
• Gene Symbol: Fabp5
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: FABP5
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins