Invitrogen™ Human Fc epsilon RI ELISA Kit

Catalog No.	Quantity
BMS21012TEN	10 x 96 Tests
BMS21012	96 Tests

Catalog No. BMS21012TEN Supplier Invitrogen™ Supplier No. BMS21012TEN

Description

ELISA

FceR1 (Fc epsilonR1 alpha) is a subunit of the high affinity receptor of IgE. Fc epsilonR1 alpha is a tetrameric complex consisting of one alpha, one ß and two additional subunits. The latter two are required for signal transduction activity. The Fc epsilonR1 complex plays an important role in triggering allergic responses, and the immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine, which are responsible for allergy symptoms, are released. The IgE receptor couples allergen and mast cells to initiate the inflammatory responses that are characteristic of disorders such as hay fever and asthma. The release of histamine and proteases leads to the synthesis of prostaglandins and leukotrienes-potent effectors of the hypersensitivity response.

Specifications

• Accession Number: P12319
• Assay Range: 0.078 to 5 ng/mL
• Assay Sensitivity: <0.068 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 2205
• Gene Symbol: FCER1A
• Interassay CV: 6.8%
• Intraassay CV: 6.1
• Kit Contents: Pre-coated 96 well plate, Standard, Detection antibody (chimeric IgE), IgE - HRP conjugate, Assay Buffer concentrate, Wash Buffer concentrate, Substrate solution, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 20 μL, Serum 20 μL, Supernatant 20 μL
• Storage Requirements: 2°C to 8°C
• Target: FceR1 alpha
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 30 mins