Invitrogen™ Human Ferritin ELISA Kit

Catalog No.	Quantity
EEL168	96 Tests

Catalog No. EEL168 Supplier Invitrogen™ Supplier No. EEL168

Description

ELISA

The Human Ferritin (FE) ELISA quantitates FE in serum, plasma and other biological fluids. Principle of the method The Human Ferritin (FE) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. Ferritin is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in the light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. The ferritin gene has multiple pseudogenes, and ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis. Ferritin is a holoenzyme shell with a molecular weight of about 450 kDa, and consists of 24 subunits of two types, H(heavy) and L(light), and capable of storing up to 4,500 atoms of ferric iron.

Specifications

• Accession Number: P02792, P02794
• Assay Range: 0.16-10 ng/mL
• Assay Sensitivity: 0.1 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: apoferritin; cell proliferation-inducing gene 15 protein; Ferritin H subunit; ferritin heavy chain; ferritin heavy chain 1; Ferritin heavy chain, N-terminally processed; Ferritin L subunit; ferritin L-chain; ferritin light chain; ferritin light polypeptide-like 3; Ferritin subunit H; ferritin, heavy polypeptide 1; ferritin, light polypeptide; FHC; frih; fril; FTH; Fth1; FTHL6; FTL; HFE5; H-ferritin; HFt; L apoferritin; LFTD; MFH; MGC71996; NBIA3; OK/SW-cl.84; PIG15; placenta immunoregulatory factor; PLIF; proliferation-inducing protein 15apoferritin; cell proliferation-inducing gene 15 protein; Ferritin H subunit; ferritin heavy chain; ferritin heavy chain 1; Ferritin heavy chain, N-terminally processed; Ferritin L subunit; ferritin L-chain; ferritin light chain; ferritin light polypeptide-like 3; Ferritin subunit H; ferritin, heavy polypeptide 1; ferritin, light polypeptide; FHC; frih; fril; FTH; Fth1; FTHL6; FTL; HFE5; H-ferritin; HFt; L apoferritin; LFTD; MFH; MGC71996; NBIA3; OK/SW-cl.84; PIG15; placenta immunoregulatory factor; PLIF; proliferation-inducing protein 15;
• Gene ID (Entrez): 2495, 2512
• Gene Symbol: FTH1, FTL
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Tissue Homogenate 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Ferritin
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min