Invitrogen™ Human Ferritin Light Polypeptide ELISA Kit

Catalog No.	Quantity
EEL182	96 Tests

Catalog No. EEL182 Supplier Invitrogen™ Supplier No. EEL182

Description

ELISA

The Human Ferritin, Light Polypeptide (FTL) ELISA quantitates FTL in serum, plasma and other biological fluids. Principle of the method The Human Ferritin, Light Polypeptide (FTL) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes.

Specifications

• Accession Number: P02792
• Assay Range: 3.13-200 ng/mL
• Assay Sensitivity: 1.88 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: ferritin L subunit; Ferritin L subunit 1; ferritin L-chain; ferritin light chain; Ferritin light chain 1; ferritin light polypeptide-like 3; ferritin, light polypeptide; fril; Ftl; Ftl1; Ftl-1; L-ferritin; LFTD; NBIA3ferritin L subunit; Ferritin L subunit 1; ferritin L-chain; ferritin light chain; Ferritin light chain 1; ferritin light polypeptide-like 3; ferritin, light polypeptide; fril; Ftl; Ftl1; Ftl-1; L-ferritin; LFTD; NBIA3;
• Gene ID (Entrez): 2512
• Gene Symbol: FTL
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Tissue Homogenate 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Ferritin Light Chain
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min