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Gibco™ Human FGF-10 Recombinant Protein

Catalog No. PHG0204
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25 μg
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PHG0204 25 μg
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Recombinant Protein

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 5 mM sodium phosphate, pH 7.4, to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in aqueous buffers supplemented with carrier protein, such as 0.1-1.0% BSA Storage: The lyophilized protein is stable at room temperature for up to 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with carrier protein are stable for at least 3 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

FGF10 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This protein is also implicated to be a primary factor in the process of wound healing.
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Specifications

Accession Number O15520
For Use With (Application) Bioactivity
Formulation 5mM sodium phosphate with 80mM NaCl and no preservative
Gene ID (Entrez) 2255
Molecular Weight (g/mol) 19.3 kDa
Name Human FGF-10
Purification Method Purified
Quantity 25 μg
Storage Requirements -20°C
Regulatory Status RUO
Endotoxin Concentration <0.1 ng/μg
Gene Alias AEY17; BB213776; FGF; Fgf10; FGF-10; Fibroblast growth factor; Fibroblast growth factor 10; Gsfaey17; Keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria
Common Name FGF10
Gene Symbol FGF10
Biological Activity ED50 = 0.5 - 15 ng/mL; determined by the dose-dependent proliferation of 4 MBr-5 cells.
Product Type Protein
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Sequence Human FGF-10 recombinant protein contains 170 amino acids
Content And Storage -20°C
Activity ED50 = 0.5 - 15 ng/mL; determined by the dose-dependent proliferation of 4MBr-5 cells.
Endotoxin Level <0.1 ng/μg
Shipping Condition Approved for shipment on Wet or Dry Ice
Expression System E. coli
Protein Family Growth Factors & Receptors
Protein Form Recombinant, Ligand
Form Lyophilized
Protein Subtype FGFs (Fibroblast Growth Factors)
Research Category Neurobiology, Oncology, Stem Cell Research, Microbiology, Differentiation, Angiogenesis
Product Line Gibco
Purity or Quality Grade ≥95% by SDS-PAGE and HPLC
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