Gibco™ Human FGF-4 Recombinant Protein

Catalog No.	Quantity
PHG0154	25 μg

Catalog No. PHG0154 Supplier Gibco™ Supplier No. PHG0154

Description

Recombinant Protein

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile, distilled water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C. Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage, preferably desiccated. Working aliquots stored with a carrier protein are stable for at least 3 months at -80°C to -20°C. Avoid repeated freeze/thaw cycles.

Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.

Specifications

• Accession Number: P08620
• For Use With (Application): Bioactivity
• Formulation: 10mM sodium phosphate with 100mM NaCl and no preservative; pH 7.4
• Gene ID (Entrez): 2249
• Molecular Weight (g/mol): 19.7 kDa
• Name: Human FGF-4
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 25 μg
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 ng/μg
• Gene Alias: FGF; FGF4; Fgf-4; Fgfk; Fibroblast growth factor; fibroblast growth factor 4; fibroblast growth factor 4 splice isoform; HBGF-4; heparin secretory transforming protein 1; heparin secretory-transforming protein 1; heparin-binding growth factor 1; heparin-binding growth factor 4; HST; Hst1; hst-1; HSTF1; HSTF-1; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; Kfgf; K-FGF; K-fibroblast growth factor; KS3; oncogene HST; transforming protein KS3
• Common Name: FGF4
• Gene Symbol: Fgf4
• Biological Activity: ED50 ≤ 10 ng/mL; determined by the dose-dependent proliferation of Balb/c 3T3 cells.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: Human FGF-4 recombinant protein contains 182 amino acids
• Content And Storage: -20°C
• Activity: ED50 ≤ 10 ng/mL; determined by the dose-dependent proliferation of Balb/c 3T3 cells.
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Expression System: E. coli
• Protein Family: Growth Factors & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: FGFs (Fibroblast Growth Factors)
• Research Category: Neurobiology, Oncology, Stem Cell Research, Microbiology, Differentiation, Angiogenesis
• Product Line: Gibco
• Purity or Quality Grade: ≥95% by SDS-PAGE and HPLC

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov