Gibco™ Human FGF-basic (FGF-2/bFGF) (aa 10-155) Recombinant Protein

Description

Recombinant Protein

Carrier-Free Reconstitution: Upon initial thawing, centrifuge the vial briefly, before opening to bring the contents to the bottom. Apportion the protein into working aliquots and store at ≤-20°C. Make any further dilutions of the reconstituted protein in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA. Storage: Store the unopened vial at ≤-20°C. Upon thawing, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.

FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.

Specifications

• Accession Number: P09038
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: 20 mM citrate with no preservative; pH 5
• Gene ID (Entrez): 2247
• Molecular Weight (g/mol): 17 kDa
• Name: Human FGF-basic (FGF-2/bFGF) (aa 10-155)
• Purification Method: Sequential Chromatography
• Quantity: 100 μg
• Endotoxin Concentration: <0.1 ng/ μg
• Gene Alias: Basic fibroblast growth factor; basic fibroblast growth factor bFGF; bFGF; FGF; fgf basic; Fgf2; Fgf-2; Fgfb; FGF-b; Fibroblast growth factor; fibroblast growth factor 2; fibroblast growth factor 2 (basic); HBGF-2; Heparin-binding growth factor 2; H-FGF-b-147; H-FGF-b-154; M-FGF-b; Prostatic growth factor; prostatropin
• Common Name: FGF2
• Biological Activity: ED50 ≤ 1.0 ng/mL; determined by the dose-dependent proliferation of BALB/3T3 cells.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: PALPEDGGSG AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH IKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTD ECFFFERLES NNYNTYRSRK YTSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS
• Content And Storage: -20°C
• Endotoxin Level: <0.1 ng/μg
• Indicator Cell: Balb/3T3 cells
• Shipping Condition: Dry Ice
• Specific Activity: 1.0 x 10⁷ - 1.0 x 10⁶ units⁄mg
• Expression System: E. coli
• Protein Family: Growth Factors & Receptors
• Protein Form: Recombinant, Ligand, Truncated
• Form: Lyophilized
• Protein Subtype: FGFs (Fibroblast Growth Factors)
• Research Category: Neurobiology, Oncology, Stem Cell Research, Microbiology, Angiogenesis
• Product Line: Gibco™
• Purity or Quality Grade: ≥95% by SDS-PAGE

Safety and Handling

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