Gibco™ Human FGF-basic (FGF-2/bFGF) Recombinant Protein

Description

Recombinant Protein

Carrier-Free Reconstitution: Reconstitute in 100 μL of 10 mM Tris, pH 7.6, to yield a stock solution of 0.1 mg/mL of bFGF. To avoid loss due to adsorption, prepare dilute solutions in appropriate assay buffer containing at least 0.1% BSA just prior to use. Do not store in dilute solution. For longer term storage, aliquot into buffer containing 0.1% BSA and store in polypropylene vials at -20°C. Avoid repeated freezing and thawing. In applications requiring long-term use of this growth factor in cell cultures, refilter material after dilution in BSA-containing buffer, through a 0.22 micron low protein-binding filter Storage: Six months at -20°C as received. Up to six months at -20°C when aliquoted into solution containing carrier protein (see Reconstitution Recommendation). NOTE: Do not store in dilute aqueous solution. Avoid repeated freeze/thaw.

FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.

Specifications

• Accession Number: P09038
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 2247
• Name: Human FGF-basic (FGF-2/bFGF)
• Purification Method: PAGE
• Quantity: 10 μg
• Endotoxin Concentration: ≤0.1 ng/ μg
• Gene Alias: Basic fibroblast growth factor; basic fibroblast growth factor bFGF; bFGF; FGF; fgf basic; Fgf2; Fgf-2; Fgfb; FGF-b; Fibroblast growth factor; fibroblast growth factor 2; fibroblast growth factor 2 (basic); HBGF-2; Heparin-binding growth factor 2; H-FGF-b-147; H-FGF-b-154; M-FGF-b; Prostatic growth factor; prostatropin
• Common Name: FGF2
• Biological Activity: ED50 < 1.0 ng/mL; determined by the dose-dependent stimulation of BALB/3T3 cells.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Content And Storage: -20°C
• Classification: Carrier-Free
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Dry Ice
• Expression System: E. coli
• Protein Family: Growth Factors & Receptors
• Protein Form: Full Length, Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: FGFs (Fibroblast Growth Factors)
• Research Category: Neurobiology, Oncology, Stem Cell Research, Microbiology, Differentiation, Angiogenesis
• Purity or Quality Grade: ≥95% by SDS-PAGE

Safety and Handling

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