Invitrogen™ Human Ficolin-3 ELISA Kit

Catalog No.	Quantity
EH193RBX5	5 x 96 Tests
EH193RB	96 Tests
EH193RBX10	10 x 96 Tests

Catalog No. EH193RBX5 Supplier Invitrogen™ Supplier No. EH193RBX5

Description

ELISA

Human Ficolin-3 quantitates human Ficolin-3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Ficolin-3.

H-Ficolin (Hakata antigen, Ficolin-3) is an innate immunity pattern recognition molecule found in human serum. H-Ficolin binds to distinct pathogen-associated molecular patterns (PAMP) formed by carbohydrates (GlcNAc, GalNAc and fucose) activating complement through the lectin pathway by means of the associated MASPs ((MBL)-associated serine proteases). H-Ficolin consists of 35 kDa protein chains associated in triplet subunits which are again associated in high molecular weight oligomers by means of disulfide bonds at the N-terminal region.

Specifications

• Accession Number: O75636
• Assay Range: 0.25-50 ng/mL
• Assay Sensitivity: 0.25 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Collagen/fibrinogen domain-containing lectin 3 p35; Collagen/fibrinogen domain-containing protein 3; FCN3; FCNH; ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen); ficolin 3; ficolin-3; HAKA1; Hakata antigen; LOW QUALITY PROTEIN: ficolin-3; MGC22543Collagen/fibrinogen domain-containing lectin 3 p35; Collagen/fibrinogen domain-containing protein 3; FCN3; FCNH; ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen); ficolin 3; ficolin-3; HAKA1; Hakata antigen; LOW QUALITY PROTEIN: ficolin-3; MGC22543;
• Gene ID (Entrez): 8547
• Gene Symbol: FCN3
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: Ficolin 3
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min