Invitrogen™ Human Gastrin ELISA Kit

Catalog No.	Quantity
EEL058	96 Tests

Catalog No. EEL058 Supplier Invitrogen™ Supplier No. EEL058

Description

ELISA

The Human Gastrin (GT) ELISA quantitates GT in serum, plasma and other biological fluids Principle of the method: The Competitive Gastrin (GT) ELISA research-use-only kit is designed to quantitatively measure GT in Humans. A GT standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Human GT is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Human GT in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This antibody may be used to study the differential tissue expression and intracellular and subcellular localization of gastrin in neuroendocrine cells of the gastrointestinal tract, and in the CNS. Antibodies to gastrin are also useful for the identification and detection of gastrin in normal and neoplastic tissue.

Specifications

• Accession Number: P01350
• Assay Range: 15.63-1000 pg/mL
• Assay Sensitivity: 9.38 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Gastrin/cholecystokinin
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Big gastrin; G14; G17; G34; G52; G6; G71; GAS; Gast; Gastrin; Gastrin component I; Gastrin component II; Gastrin component III; gastrin-1; Gastrin-14; Gastrin-17; Gastrin-34; Gastrin-52; Gastrin-6; Gastrin-71; PPG34; preprogastrinBig gastrin; G14; G17; G34; G52; G6; G71; GAS; Gast; Gastrin; Gastrin component I; Gastrin component II; Gastrin component III; gastrin-1; Gastrin-14; Gastrin-17; Gastrin-34; Gastrin-52; Gastrin-6; Gastrin-71; PPG34; preprogastrin;
• Gene ID (Entrez): 2520
• Gene Symbol: Gast
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Gastrin
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min