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Proteintech Group Inc Human GFAP ELISA Kit
Supplier: Proteintech Group Inc KE0021096T
KE00210 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GFAP ELISA kit is to be used to detect and quantify protein levels of endogenous GFAP. The assay recognizes human GFAP. An antibody specific for GFAP has been pre-coated onto the microwells. The GFAP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for GFAP is added to detect the captured GFAP protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .
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