Invitrogen™ Human GFR alpha-1/GDNF R ELISA Kit

Description

ELISA

Human GFR alpha-1/GDNF R quantitates human GFR alpha-1/GDNF R in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human GFR alpha-1/GDNF R.

Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nerves system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFRalpha, which is a cell surface receptor for GDNF. The cDNAs encoding GDNFRalpha from human, rat, chicken and mouse have been cloned recently. GDNFRalpha was also termed Ret ligand 1 (RETL1) or TGF-beta-related neurotrophic factor receptor 1 (TrnR1) and nominated as GFR-a-1 recently. GFR-a-1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFR-a and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function.

Specifications

• Accession Number: AU042498; DKFZp313E1012; DKFZp686J0156; FLJ10561; FLJ31546; GDNF family receptor alpha 1; GDNF family receptor alpha-1; GDNF receptor alpha; GDNF receptor alpha-1; GDNFR; GDNFRA; GDNFR-alpha; GDNFR-alpha-1; gfr alpha 1; GFR alpha-1; GFRA1; GFR-alpha-1; glial cell line derived neurotrophic factor family receptor alpha 1; Glial cell line-derived neurotrophic factor receptor alpha; GPI-linked anchor protein; MGC23045; PI-linked cell-surface accessory protein; RET ligand 1; RET1L; RETL1; TGF-beta-related neurotrophic factor receptor 1; Trnr1
• Assay Range: 0.35 to 80 ng/mL
• Assay Sensitivity: 0.35 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 2674
• Gene Symbol: GFRA1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: GFR alpha-1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov