ACROBiosystems ACROBiosystems Human Glypican 3 / GPC3 Protein, His Tag (MALS verified)

Supplier:  ACROBiosystems GP3H52H4100UG

Recombinant Protein;100UG;Human Glypican 3, His Tag (GP3-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1).;This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 24.6 kDa respectively. The protein migrates as 40 kDa, 32 kDa and 66-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human Glypican 3, His Tag (Cat. No. GP3-H52H4) at 0.5 μg/mL (100 μL/well) can bind Anti-Human GPC3 MAb with a linear range of 0.1-2 ng/mL (QC tested).;Anti-human GPC3 MAb (chimeric mouse-human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human Glypican 3, His Tag (Cat. No. GP3-H52H4) with an affinity constant of 1.33 nM as determined in a SPR assay (Biacore T200) (Routinely tested).;2e5 of anti-GPC3 CAR-293 cells w

Catalog No. 50-210-6653