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Invitrogen™ eBioscience™ Human GM-CSF ELISA Ready-SET-Go!™ Kit, 2nd Generation Non-distribution product as customer accommodation.

Specifically engineered for accurate and precise measurement protein levels from samples including serum, plasma, and cell culture supernatants. This second generation kit has increased sensitivity, with a standard curve range from 6-750pg/mL.

Manufacturer:  Invitrogen™ 88833722

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Catalog No. 50-112-5221


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Alert:

Contains Sodium azide


Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer . Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent . Buffer for blocking and diluting the Detection Antibody and Enzyme
Enzyme. Pre-titrated Avidin-HRP
Substrate. 1X TMB Solution

Description

Description

GM-CSF (Granulocyte-Macrophage colony-stimulating factor) is a 14.6kDa hematopoietic growth factor that exists in glycosylated and non-glycosylated biologically active forms, and stimulates the development of granulocytes, macrophages, early megakaryocytes and eosinophil progenitor cells. The active form of the GM-CSF protein is found extracellularly as a homodimer and the GM-CSF gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q-syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. The ability of recombinant GM-CSF to increase hematopoietic cell recovery has become a focus area in the therapeutic treatment of patients following bone marrow transplantation. Recent studies have investigated GM-CSF in inflammatory and autoimmune diseases such as arthritis, arthritis related interstitial lung disease, nephritis, and psoriasis. In the CNS, GM-CSF depletion or neutralization has been studied in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS).
Specifications

Specifications

P04141
Biotin
1437
Colorimetric Microplate Reader
ELISA Kit
RUO
Plasma,Serum,Supernatant
Human
1 hr 45 min
Human
Granulocyte Macrophage Colony-Stimulating Factor, CSF2
6-750 pg/mL
ELISA
96-well plates
10X Coating Buffer: Buffer for plating the Capture Antibody
2 x 96 tests
6 pg/mL
Plasma,100 μL|Serum,100 μL|Supernatant,100 μL
2-8°C
24 hr 30 min
CSF2
HRP
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