Invitrogen™ Human GM-CSF Uncoated ELISA Kit with Plates

Catalog No.	Quantity
50-112-5221	2 x 96 Tests

Catalog No. 50-112-5221 Supplier Invitrogen™ Supplier No. 88833722

Description

ELISA

The Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human GM-CSF. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.

Specifications

• Accession Number: P04141
• Assay Range: 6-750 pg/mL
• Assay Sensitivity: 6 pg/mL
• Content And Storage: Capture Antibody: Pre-titrated, purified antibody
  Detection Antibody: Pre-titrated, biotin-conjugated antibody
  Standard: Recombinant protein for generating standard curve and calibrating samples
  10X Coating Buffer: Buffer for plating the Capture Antibody
  5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme
  Enzyme: Pre-titrated Avidin-HRP
  Substrate: 1X TMB Solution
  Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
  96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22, -76, -86)
• Conjugate: HRP
• Immunoassay Kit Format: Uncoated ELISA Kit with Plates
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: colony stimulating factor 2; colony stimulating factor 2 (granulocyte-macrophage); colony-stimulating factor; CSF; CSF2; Csfgm; cytokine; GMCSF; Gm-CSf; granulocyte macrophage colony stimulating factor; granulocyte macrophage-colony stimulating factor; granulocyte-macrophage colony stimulating factor 2; granulocyte-macrophage colony-stimulating factor; MGC131935; MGC138897; MGC151255; MGC151257; MGI-IGM; M-GM-CSF; molgramostin; put. GM-CSF; sargramostimcolony stimulating factor 2; colony stimulating factor 2 (granulocyte-macrophage); colony-stimulating factor; CSF; CSF2; Csfgm; cytokine; GMCSF; Gm-CSf; granulocyte macrophage colony stimulating factor; granulocyte macrophage-colony stimulating factor; granulocyte-macrophage colony stimulating factor 2; granulocyte-macrophage colony-stimulating factor; MGC131935; MGC138897; MGC151255; MGC151257; MGI-IGM; M-GM-CSF; molgramostin; put. GM-CSF; sargramostim; CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF; NKSF2;CLMF; IL-12A; NFSK; NKSF1; P35
• Gene ID (Entrez): 1437
• Gene Symbol: CSF2
• Kit Contents: Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86), Purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
• Label or Dye: Biotin
• Protein Aliases: GM-CSF, Colony-stimulating factor, CSF, Molgramostin, Sargramostim
• Quantity: 2 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
• Storage Requirements: 2-8°C
• Synonym: Granulocyte Macrophage Colony-Stimulating Factor, CSF2
• Target: GM-CSF
• Test Time: 1 hr 45 min
• Total Assay Time: 24 hr 30 min