Invitrogen™ Human GRO beta (CXCL2) ELISA Kit

Catalog No.	Quantity
EEL054	96 Tests

Catalog No. EEL054 Supplier Invitrogen™ Supplier No. EEL054

Description

ELISA

The Human Growth Regulated Oncogene Beta (GROB/CXCL2) ELISA quantitates GROB/CXCL2 in serum, plasma, and other biological fluids The Human GROB/CXCL2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.

Specifications

• Accession Number: P19875
• Assay Range: 15.63-1000 pg/mL
• Assay Sensitivity: 9.38 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: CXC chemokine
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 2; CINC-2a; Cinc3; CINC-3; CXC; CXC motif chemokine; C-X-C motif chemokine 2; C-X-C motif chemokine ligand 2; CXCL; CXCL2; Cytokine-induced neutrophil chemoattractant 3; GRO b; gro beta; GRO β; GRO2; GRO2 oncogene; GROb; GRObeta; gro-beta; GRO-beta(5-73); GRO-beta-T; Growth-regulated protein beta; GROβ; Hematopoietic synergistic factor; HSF; Macrophage inflammatory protein 2; macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; Mgsa-b; MIP 2 a; MIP 2 alpha; MIP 2 α; Mip2; MIP-2; MIP2 a; MIP2 α; MIP2A; MIP-2a; MIP2-alpha; SB-251353; Scyb; Scyb2; small inducible cytokine subfamily, member 2C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 2; CINC-2a; Cinc3; CINC-3; CXC; CXC motif chemokine; C-X-C motif chemokine 2; C-X-C motif chemokine ligand 2; CXCL; CXCL2; Cytokine-induced neutrophil chemoattractant 3; GRO b; gro beta; GRO β; GRO2; GRO2 oncogene; GROb; GRObeta; gro-beta; GRO-beta(5-73); GRO-beta-T; Growth-regulated protein beta; GROβ; Hematopoietic synergistic factor; HSF; Macrophage inflammatory protein 2; macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; Mgsa-b; MIP 2 a; MIP 2 alpha; MIP 2 α; Mip2; MIP-2; MIP2 a; MIP2 α; MIP2A; MIP-2a; MIP2-alpha; SB-251353; Scyb; Scyb2; small inducible cytokine subfamily, member 2;
• Gene ID (Entrez): 2920
• Gene Symbol: Cxcl2
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: CXCL2
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min