Invitrogen™ Human GSTM1 ELISA Kit

Description

ELISA

Human GSTM1 quantitates human GSTM1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human GSTM1.

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13. 3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene.

Specifications

• Accession Number: glutathione S-alkyltransferase; glutathione S-aralkyltransferase; glutathione S-aryltransferase; Glutathione S-transferase GT8.7; glutathione S-transferase M1; glutathione S-transferase Mu 1; Glutathione S-transferase Yb-1; glutathione S-transferase Yb-1 subunit; glutathione S-transferase, mu 1; glutathione-S-transferase mu type 1 (Yb1); glutathione-S-transferase, mu 1; glutathione-S-transferase, mu type 1 (Yb1); GST 1-1; GST 3-3; GST class-mu 1; GST HB subunit 4; GST Yb1; GST1; GSTA3; Gstb1; Gstb-1; GSTM1; GSTM1-1; GSTM1a-1 A; GSTM1b-1 b; GTH4; GTM1; H-B; HB subunit 4; MU; MU-1; pmGT10; S-(hydroxyalkyl)glutathione lyase
• Assay Range: 2 to 500 ng/mL
• Assay Sensitivity: 2 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 2944
• Gene Symbol: GSTM1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: GSTM1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins