Gibco™ Human Heat Stable bFGF Recombinant Protein

Description

Recombinant Protein

Carrier-Free Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water to a concentration of 1.0 mg/mL. Alternatively, reconstitute at lower concentrations in carrier-containing medium. Do not vortex. Apportion the reconstituted protein into working aliquots and store at -20°C. Make any further dilutions of the reconstituted protein in medium, or buffered solution containing carrier protein, such as PBS with 0.1% BSA. For the 5 μg unit size, reconstitute directly in carrier-containing medium. For other unit sizes, reconstitute to 1.0 mg/mL as follows: , 50 μg, reconstitute in 50 μL, 100 μg, reconstitute in 100 μL, 500 μg, reconstitute in 500 μL Storage: Store the lyophilized protein at -20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at -20°C (not in a frost-free freezer) for up to 12 months. Avoid repeated freeze-thaw cycles.

FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.

Specifications

• Accession Number: P09038
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: 20 mM potassium phosphate with 750 mM NaCl and no preservative
• Gene ID (Entrez): 2247
• Molecular Weight (g/mol): 19 kDa
• Name: Human Heat Stable bFGF
• Quantity: 500 μg
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: Basic fibroblast growth factor; basic fibroblast growth factor bFGF; bFGF; FGF; fgf basic; Fgf2; Fgf-2; Fgfb; FGF-b; Fibroblast growth factor; fibroblast growth factor 2; fibroblast growth factor 2 (basic); HBGF-2; Heparin-binding growth factor 2; H-FGF-b-147; H-FGF-b-154; M-FGF-b; Prostatic growth factor; prostatropin
• Common Name: FGF2
• Protein Length: 155 aa + 20 aa for N-terminal tag for purification purposes
• Biological Activity: ED50 = 0.6 - 1.1 ng/mL; determined by the dose-dependent proliferation of mouse NIH/3T3 fibroblast cells.
• Product Type: Heat Stable Recombinant Human bFGF
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sterility: Sterile
• Content And Storage: -20°C
• Classification: Carrier-Free
• Endotoxin Level: <1 EU/μg
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Expression System: E. coli
• Protein Family: Growth Factors and Receptors
• Form: Lyophilized
• Research Category: Neurobiology, Oncology, Stem Cell Research, Microbiology, Differentiation, Angiogenesis
• Purity or Quality Grade: >95% by SDS-PAGE

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov