Gibco™ Human Heat Stable FGF-basic (FGF-2/bFGF) (154 aa) Recombinant Protein, PeproTech®

Description

Recombinant Protein

FGF-basic loses its activity over time in solution at physiological temperatures and needs to be re-supplemented periodically to maintain its performance. As a result of selected amino acid substitutions, Heat Stable FGF-basic retains full biological activity and also maintains full activity over extended periods of time in solution at 370 C. These properties make Heat Stable FGF-basic a superior choice for use in cell culture media applications. Recombinant Heat Stable FGF-basic is a 17.2 kDa protein containing 154 amino acids. Human FGF-basic (FGF-2/bFGF) (154 aa), Recombinant Protein PeproTech® was developed under a license from Enantis. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.

Specifications

• Accession Number: P09038
• For Use With (Application): Functional Assay
• Format: Lyophilized
• Formulation: protein with no preservative
• Gene ID (Entrez): 2247
• Molecular Weight (g/mol): 17.2 kDa
• Name: Human Heat Stable FGF-basic (FGF-2/bFGF) (154 aa)
• Purification Method: HPLC, SDS-PAGE
• Quantity: 10 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: Basic fibroblast growth factor; basic fibroblast growth factor bFGF; bFGF; FGF; fgf basic; Fgf2; Fgf-2; Fgfb; FGF-b; Fibroblast growth factor; fibroblast growth factor 2; fibroblast growth factor 2 (basic); HBGF-2; Heparin-binding growth factor 2; H-FGF-b-147; H-FGF-b-154; M-FGF-b; Prostatic growth factor; prostatropin
• Common Name: FGF2
• Gene Symbol: FGF2
• Biological Activity: Determined by a cell proliferation assay using Balb/c 3t3 cells. The expected ED50 range is 0.05-0.25 ng/ml
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: Thermostable variant of human FGF-basic (154 amino acids) with proprietary amino acid substitutions.
• Content And Storage: -20°C
• Activity: Determined by a cell proliferation assay using Balb/c 3t3 cells. The expected ED50 range is 0.05-0.25 ng/ml
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95% by SDS-PAGE gel and HPLC analyses.