Invitrogen™ Human Hemoglobin ELISA Kit

Catalog No.	Quantity
EH237RB	96 Tests
EH237RBX5	5 x 96 Tests

Catalog No. EH237RB Supplier Invitrogen™ Supplier No. EH237RB

Description

ELISA

Human Hemoglobin quantitates human Hemoglobin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Hemoglobin.

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.

Specifications

• Accession Number: P68871, P69905
• Assay Range: 1.5-400 ng/mL
• Assay Sensitivity: 1.5 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: alpha one globin; alpha-1 globin; alpha-1-globin; alpha-2 globin chain; Alpha-globin; ALPHA-GLOBIN GENE; delta globin; Haemoglobin; Hb; HBA1; HbA1c; HBA2; HBA-T3; HBH; hemoglobin alpha 1 globin chain; Hemoglobin alpha chain; Hemoglobin subunit alpha; hemoglobin subunit alpha 1; hemoglobin, alpha 1alpha one globin; alpha-1 globin; alpha-1-globin; alpha-2 globin chain; Alpha-globin; ALPHA-GLOBIN GENE; delta globin; Haemoglobin; Hb; HBA1; HbA1c; HBA2; HBA-T3; HBH; hemoglobin alpha 1 globin chain; Hemoglobin alpha chain; Hemoglobin subunit alpha; hemoglobin subunit alpha 1; hemoglobin, alpha 1;
• Gene ID (Entrez): 3039, 3043
• Gene Symbol: HBA1, HBB
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: Hemoglobin
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min